low aoc3  (R&D Systems)

Journal: Communications Biology

Article Title: Crosstalk of cell death pathways unveils an autophagy-related gene AOC3 as a critical prognostic marker in colorectal cancer

doi: 10.1038/s42003-024-05980-6

Figure Lengend Snippet: a Univariate Cox analysis of CDN4 feature genes with p < 0.05 and HR > 1 in TCGA and GEO prognosis cohorts. b Mean C-index of the candidate genes. c – f Kaplan–Meier curves of overall survival with the log-rank test between the high AOC3 group and low AOC3 group in TCGA and three validation datasets. g Representative immunohistochemistry (IHC) staining images of CRC tissue microarrays in low AOC3 tumor expression and high AOC3 tumor expression tissues. h Kaplan–Meier curves of overall survival with the log-rank test between the high AOC3 H-scores group and low AOC3 H-scores group in IHC arrays. i The molecules significantly associated with AOC3 were enriched in regulation of autophagy pathway. j Correlation analysis of AOC3 with regulation of autophagy pathways score. k Differential expression of autophagy genes in different AOC3 expressions. ns fdr>0.05, *fdr < 0.05, **fdr < 0.01, ***fdr < 0.001, ****fdr < 0.0001.

Article Snippet: Immunohistochemistry (IHC) was performed using AOC3 (1:600; Cat no. 66834-1-Ig, Proteintech, Wuhan, China).

Techniques: Biomarker Discovery, Immunohistochemistry, Expressing, Quantitative Proteomics

Journal: Journal of Cellular and Molecular Medicine

Article Title: Single‐cell transcriptional profiling of human carotid plaques reveals a subpopulation of endothelial cells associated with stroke incidences

doi: 10.1111/jcmm.17354

Figure Lengend Snippet: AOC3 is related to plaque haemorrhage and plaque stability. (A) Venn plot showing the overlapping relationships of up‐regulated genes about cell adhesion in ASYM, stable and non‐IPH group. (B) Violin plots of AOC3 expression in all three EC subpopulations. (C) The AOC3 expression in stable plaques ( n = 4) and unstable plaques ( n = 4). (D) The AOC3 expression in non‐IPH ( n = 16) and IPH ( n = 27) group. (E) Gene ontology enrichment analysis of KEGG pathway with up‐regulated genes in EC 2 subcluster of ASYM group. (F) A protein interaction network was constructed using all differentially expressed genes in the EC 2 subcluster. Red colour represents up‐regulated genes, and green colour represents down‐regulated genes. ASYM, asymptomatic carotid artery plaque; EC, endothelial cells; IPH, intraplaque haemorrhage

Article Snippet: After antigen retrieval with citrate buffer (P0088, Beyotime), the sections were incubated with an anti‐human AOC3 antibody (1:200, 14365‐1‐AP, Proteintech) or a rabbit IgG control at 4°C overnight followed by incubation with an HRP‐conjugated secondary antibody (1 μg/ml, GK500705, Genetech) for 30 min.

Techniques: Expressing, Construct

Journal: Journal of Cellular and Molecular Medicine

Article Title: Single‐cell transcriptional profiling of human carotid plaques reveals a subpopulation of endothelial cells associated with stroke incidences

doi: 10.1111/jcmm.17354

Figure Lengend Snippet: AOC3 expression in carotid atherosclerotic plaques from symptomatic patients. (A) carotid intima sections were stained with haematoxylin–eosin (bar = 1000 µm). The magnified images of the atherosclerotic area (AA) and non‐plaque region (NR) are shown below (bar = 50 µm). (B) Representative immunohistochemical staining images for AOC3 in AA and NR regions. The dotted lines define the regions of the plaques. Nuclei were stained by haematoxylin (bar = 1000 µm). The magnified image was shown below, where the arrows indicated the AOC3 expression in nucleated cells of the intima (bar = 50 µm)

Article Snippet: After antigen retrieval with citrate buffer (P0088, Beyotime), the sections were incubated with an anti‐human AOC3 antibody (1:200, 14365‐1‐AP, Proteintech) or a rabbit IgG control at 4°C overnight followed by incubation with an HRP‐conjugated secondary antibody (1 μg/ml, GK500705, Genetech) for 30 min.

Techniques: Expressing, Staining, Immunohistochemical staining